ARG81523

Human CYR61 ELISA Kit

Human CYR61 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81523-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81523-002 Standard 2 X 10 ng/vial 4°C
ARG81523-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81523-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81523-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81523-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81523-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81523-008 25X Wash buffer 20 ml 4°C
ARG81523-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81523-010 STOP solution 10 ml (Ready to use) 4°C
ARG81523-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81523 Human CYR61 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CYR61 in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CYR61
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%
Inter-Assay CV: 7.0%
Alternate Names Protein GIG1; CCN1; IGFBP-10; Insulin-like growth factor-binding protein 10; IGFBP10; GIG1; Cysteine-rich angiogenic inducer 61; Protein CYR61; CCN family member 1; IGF-binding protein 10; IBP-10

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3491 Human CYR61

Swiss-port # O00622 Human Protein CYR61

Gene Symbol CYR61
Gene Full Name cysteine-rich, angiogenic inducer, 61
Background The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]
Function Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. [UniProt]
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