ARG82502
Human Carboxypeptidase A1 ELISA Kit
Human Carboxypeptidase A1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82502-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82502-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82502-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82502-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82502-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82502-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82502-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82502-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82502-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82502-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82502-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82502 Human Carboxypeptidase A1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Carboxypeptidase A1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Carboxypeptidase A1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2% Inter-Assay CV: 7.0% |
| Alternate Names | CPA; Carboxypeptidase A1; EC 3.4.17.1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CPA1 |
| Gene Full Name | carboxypeptidase A1 (pancreatic) |
| Background | This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. [provided by RefSeq, Jan 2015] |
| Function | Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Carboxypeptidase antibodies; Carboxypeptidase ELISA Kits; Carboxypeptidase recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82502 Human Carboxypeptidase A1 ELISA Kit User manual |
 Download
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