ARG81144

Human Cardiac Troponin I ELISA Kit

Human Cardiac Troponin I ELISA Kit for ELISA and Human

Overview

Product Description ARG81144 Human Cardiac Troponin I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cardiac Troponin I in serum.
Tested Reactivity Hu
Tested Application ELISA
Target Name Cardiac Troponin I
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 2.0 ng/ml
Sample Type Serum
Standard Range 2 - 75 ng/ml
Sample Volume 100 µl
Alternate Names RCM1; cTnI; Cardiac troponin I; TNNC1; CMD1FF; CMD2A; Troponin I, cardiac muscle; CMH7

Application Instructions

Assay Time ~ 2.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7137 Human TNNI3

Swiss-port # P19429 Human Troponin I, cardiac muscle

Gene Symbol TNNI3
Gene Full Name troponin I type 3 (cardiac)
Background Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Highlight Related products:
Troponin antibodies; Troponin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
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