ARG82848
Human Cathepsin E ELISA Kit
Human Cathepsin E ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82848-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82848-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82848-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82848-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82848-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82848-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82848-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82848-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82848-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82848-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82848-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82848 Human Cathepsin E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin E in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cathepsin E |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| Alternate Names | EC 3.4.23.34; CATE; Cathepsin E |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTSE |
| Gene Full Name | cathepsin E |
| Background | This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. [provided by RefSeq, Nov 2015] |
| Function | May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. [UniProt] |
| Cellular Localization | Endosome. Note=The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. [UniProt] |
| PTM | Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide. Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82848 Human Cathepsin E ELISA Kit User manual |
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