ARG82692

Human Cathepsin L ELISA Kit

Human Cathepsin L ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82692-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82692-002 Standard 2 X 10 ng/vial 4°C
ARG82692-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82692-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82692-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82692-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82692-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82692-008 25X Wash buffer 20 ml 4°C
ARG82692-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82692-010 STOP solution 10 ml (Ready to use) 4°C
ARG82692-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82692 Human Cathepsin L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin L in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Cathepsin L
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 32 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.7%
Inter-Assay CV: 5.6%
Alternate Names Major excreted protein; Cathepsin L; CATL; Cathepsin L1; EC 3.4.22.15; CTSL1; MEP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1514 Human CTSL

Swiss-port # P07711 Human Cathepsin L1

Gene Symbol CTSL
Gene Full Name cathepsin L
Background The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
Function Important for the overall degradation of proteins in lysosomes. [UniProt]
Cellular Localization Lysosome. [UniProt]
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Title Download Link
ARG82692 Human Cathepsin L ELISA kit User manual download icon Download