ARG81545
Human Corin ELISA Kit
Human Corin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81545-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81545-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81545-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81545-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81545-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81545-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81545-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81545-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81545-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81545-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81545-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81545 Human Corin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Corin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Corin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 6.9% |
| Alternate Names | Pro-ANP-converting enzyme; ATC2; Lrp4; Heart-specific serine proteinase ATC2; Transmembrane protease serine 10; Corin; TMPRSS10; EC 3.4.21.-; CRN; Atrial natriuretic peptide-converting enzyme; PEE5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y5Q5 Human Atrial natriuretic peptide-converting enzyme |
|---|---|
| Gene Symbol | CORIN |
| Gene Full Name | corin, serine peptidase |
| Background | This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| Function | Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Isoform 2: has weaker endopeptidase activity compared to isoform 1. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated; required for processing and activation. Activated through proteolytic processing by a trypsin-like protease; cleaved into a N-terminal propeptide and an activated corin protease fragment. Different soluble forms are produced by cleavage and autocatalytic cleavage: Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment is produced by cleavage by ADAM10, while 160 kDa and 100 kDa soluble fragments are produced by autocatalytic cleavage. Cleavage by ADAM10 to produce soluble 180 kDa soluble fragment takes place after the transmembrane region and before FZ 1. A disulfide bond links the activated corin protease fragment and the N-terminal propeptide. The disulfide bond also links the activated corin protease fragment with soluble fragments (100 kDa, 160 kDa and 180 kDa fragments). [UniProt] |
| Title | Download Link |
|---|---|
| ARG81545 Human Corin ELISA Kit User's manual |
 Download
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