ARG82619

Human DBP / Vitamin D binding protein ELISA Kit

Human DBP / Vitamin D binding protein ELISA Kit for ELISA and Human

Overview

Product Description ARG82619 Human DBP / Vitamin D binding protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DBP in serum, plasma, cell culture supernatants, urine, saliva, milk and cerebrospinal fluid.
Tested Reactivity Hu
Species Does Not React With Ms, Rat, Bov, Dog, Mk, Pig, Rb
Tested Application ELISA
Specificity The kit does not cross-react with the following species: Dog, Bovine, Monkey, Mouse, Rat, Pig and Rabbit.
Target Name DBP / Vitamin D binding protein
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.38 ng/ml
Sample Type Serum, plasma, cell culture supernatants, urine, saliva, milk and cerebrospinal fluid.
Standard Range 1.56 - 100 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: 4.7%
Inter-Assay CV: 9.2%
Alternate Names GRD3; DBP/GC; HEL-S-51; VDBG; VDB; Gc-globulin; DBP; VDBP; Vitamin D-binding protein; Group-specific component

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2638 Human GC

Swiss-port # P02774 Human Vitamin D-binding protein

Gene Symbol GC
Gene Full Name group-specific component (vitamin D binding protein)
Background The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
Function Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
DBP antibodies; DBP ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-linked N-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. [UniProt]
Title Download Link
ARG82619 Human DBP / Vitamin D binding protein ELISA Kit User manual download icon Download