ARG81868
Human DLL1 ELISA Kit
Human DLL1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81868-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81868-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81868-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81868-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81868-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81868-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81868-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81868-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81868-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81868-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81868-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81868 Human DLL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DLL1 in serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | DLL1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2%; Inter-Assay CV: 4.9% |
| Alternate Names | DELTA1; H-Delta-1; Drosophila Delta homolog 1; Delta-like protein 1; DL1; Delta1; Delta |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DLL1 |
| Gene Full Name | delta-like 1 (Drosophila) |
| Background | DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008] |
| Function | Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. [UniProt] |
| Highlight | Related products: DLL1 antibodies; DLL1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). Ubiquitinated; promotes recycling back to the plasma membrane and confers a strong affinity for NOTCH1. Multi-ubiquitination of LYS-613 by MIB1 promotes both cis and trans-interaction with NOTCH1, as well as activation of Notch signaling. Ubiquitinated by NEURL1B (By similarity). Phosphorylated in a membrane association-dependent manner. Phosphorylation at Ser-697 requires the presence of Ser-694, whereas phosphorylation at Ser-694 occurs independently of the other site. Phosphorylation is required for full ligand activity in vitro and affects surface presentation, ectodomain shedding, and endocytosis. O-fucosylated. Can be elongated to a disaccharide by MFNG. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81868 Human DLL1 ELISA Kit User's manual |
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