ARG81470

Human Entactin ELISA Kit

Human Entactin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81470-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81470-002 Standard 2 X 10 ng/vial 4°C
ARG81470-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81470-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81470-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81470-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81470-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81470-008 25X Wash buffer 20 ml 4°C
ARG81470-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81470-010 STOP solution 10 ml (Ready to use) 4°C
ARG81470-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81470 Human Entactin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Entactin in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Entactin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.5%
Alternate Names Entactin; NID-1; Nidogen-1; NID

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4811 Human NID1

Swiss-port # P14543 Human Nidogen-1

Gene Symbol NID1
Gene Full Name nidogen 1
Background This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]
Function Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. [UniProt]
Highlight Related products:
Entactin antibodies; Entactin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N- and O-glycosylated. [UniProt]
Title Download Link
ARG81470 Human Entactin ELISA Kit User's manual download icon Download