ARG81733

Human EpCAM ELISA Kit

Human EpCAM ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81733-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81733-002 Standard 2 X 10 ng/vial 4°C
ARG81733-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81733-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81733-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81733-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81733-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81733-008 25X Wash buffer 20 ml 4°C
ARG81733-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81733-010 STOP solution 10 ml (Ready to use) 4°C
ARG81733-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81733 Human EpCAM ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human EpCAM in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name EpCAM
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.2%
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Highlight Related products:
EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; EpCAM recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]
Title Download Link
ARG81733 Human EpCAM ELISA Kit User's manual download icon Download