ARG70150

Human EpCAM recombinant protein (Active) (His-tagged, C-ter)

Human EpCAM recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human EpCAM recombinant protein
Tested Application SDS-PAGE
Target Name EpCAM
Species Human
A.A. Sequence MQEEC VCENY KLAVN CFVNN NRQCQ CTSVG AQNTV ICSKL AAKCL VMKAE MNGSK LGRRA KPEGA LQNND GLYDP DCDES GLFKA KQCNG TSMCW CVNTA GVRGS KLGRR AKPEG ALQNN DGLYD PDCDE SGLFK AKQCN GTSMC WCVNT AGVRS ILYEN NVITI DLVQN SSQKT QNDVD IADVA YYFEK DVKGE SLFHS KKMDL TVNGE QLDLD PGQTL IYYVD EKAPE FSMQG LK with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by the ability of the immobilized protein to support the adhesion of the 3T3 cells. The ED50 for this effect is 0.2-1.7 ng/ml.
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Cellular Localization Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction. [UniProt]
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]