ARG82624

Human FABP2 / Intestinal FABP ELISA Kit

Human FABP2 / Intestinal FABP ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82624-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82624-002 Standard 2 X 10 ng/vial 4°C
ARG82624-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82624-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82624-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82624-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82624-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82624-008 25X Wash buffer 20 ml 4°C
ARG82624-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82624-010 STOP solution 10 ml (Ready to use) 4°C
ARG82624-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82624 Human FABP2 / Intestinal FABP ELISA KitPPPPP is an Enzyme Immunoassay kit for the quantification of Human FABP2 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name FABP2 / Intestinal FABP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.3 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 7.2%
Alternate Names Intestinal-type fatty acid-binding protein; I-FABP; Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; FABPI

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2169 Human FABP2

Swiss-port # P12104 Human Fatty acid-binding protein, intestinal

Gene Symbol FABP2
Gene Full Name fatty acid binding protein 2, intestinal
Background The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]
Function FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
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Title Download Link
ARG82624 Human FABP2 / Intestinal FABP ELISA Kit User manual download icon Download