ARG81539

Human FCN1 ELISA Kit

Human FCN1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81539-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81539-002 Standard 2 X 50 ng/vial 4°C
ARG81539-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81539-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81539-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81539-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81539-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81539-008 25X Wash buffer 20 ml 4°C
ARG81539-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81539-010 STOP solution 10 ml (Ready to use) 4°C
ARG81539-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81539 Human FCN1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FCN1 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name FCN1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%
Inter-Assay CV: 6.2%
Alternate Names Ficolin-A; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-1; M-ficolin; FCNM

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2219 Human FCN1

Swiss-port # O00602 Human Ficolin-1

Gene Symbol FCN1
Gene Full Name ficolin (collagen/fibrinogen domain containing) 1
Background The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008]
Function Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. [UniProt]
Highlight Related products:
FCN1 antibodies; FCN1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81539 Human FCN1 ELISA Kit User's manual download icon Download