ARG70108

Human FGF acidic recombinant protein (Active) (His-tagged, C-ter)

Human FGF acidic recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human FGF acidic recombinant protein.
Tested Application SDS-PAGE
Target Name FGF acidic
Species Human
A.A. Sequence MFNLP PGNYK KPKLL YCSNG GHFLR ILPDG TVDGT RDRSD QHIQL QLSAE SVGEV YIKST ETGQY LAMDT DGLLY GSQTP NEECL FLERL EENHY NTYIS KKHAE KNWFV GLKKN GSCKR GPRTH YGQKA ILFLP LPVSS D with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.3 ng/ml. The specific activity of recombinant human FGF-1 is > 1 x 10^6 IU/mg.
Alternate Names HBGF1; FGF-1; FGF-alpha; GLIO703; ECGFA; ECGFB; ECGF; Endothelial cell growth factor; FGFA; Acidic fibroblast growth factor; Heparin-binding growth factor 1; Fibroblast growth factor 1; HBGF-1; AFGF; aFGF; ECGF-beta

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FGF1
Gene Full Name fibroblast growth factor 1 (acidic)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
Cellular Localization Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Note=Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. [UniProt]
PTM In the nucleus, phosphorylated by PKC/PRKCD. [UniProt]