ARG70116
Human FGF10 recombinant protein (Active) (His-tagged, C-ter)
Human FGF10 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF10 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF10 |
| Species | Human |
| A.A. Sequence | MLGQD MVSPE ATNSS SSSFS SPSSA GRHVR SYNHL QGDVR WRKLF SFTKY FLKIE KNGKV SGTKK ENCPY SILEI TSVEI GVVAV KAINS NYYLA MNKKG KLYGS KEFNN DCKLK ERIEE NGYNT YASFN WQHNG RQMYV ALNGK GAPRR GQKTR RKNTS AHFLP MVVHS with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 8 ng/ml. The specific activity of recombinant human FGF-10 is > 1.2 x 10^5 IU/mg. |
| Alternate Names | Fibroblast growth factor 10; Keratinocyte growth factor 2; FGF-10 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF10 |
|---|---|
| Gene Full Name | fibroblast growth factor 10 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
