ARG70119
Human FGF12 recombinant protein (Active) (His-tagged, C-ter)
Human FGF12 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF12 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF12 |
| Species | Human |
| A.A. Sequence | MESKE PQLKG IVTRL FSQQG YFLQM HPDGT IDGTK DENSD YTLFN LIPVG LRVVA IQGVK ASLYV AMNGE GYLYS SDVFT PECKF KESVF ENYYV IYSST LYRQQ ESGRA WFLGL NKEGQ IMKGN RVKKT KPSSH FVPKP IEVCM YREPS LHEIG EKQGR SRKSS GTPTM NGGKV VNQDS T with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2 ng/ml. |
| Alternate Names | Fibroblast growth factor 12; FHF-1; FHF1; Myocyte-activating factor; FGF12B; Fibroblast growth factor homologous factor 1; FGF-12 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF12 |
|---|---|
| Gene Full Name | fibroblast growth factor 12 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Probably involved in nervous system development and function. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
