ARG70121
Human FGF14 recombinant protein (Active) (His-tagged, N-ter)
Human FGF14 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human FGF14 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF14 |
| Species | Human |
| A.A. Sequence | AAAIA SGLIR QKRQA REQHW DRPSA SRRRS SPSKN RGLCN GNLVD IFSKV RIFGL KKRRL RRQDP QLKGI VTRLY CRQGY YLQMH PDGAL DGTKD DSTNS TLFNL IPVGL RVVAI QGVKT GLYIA MNGEG YLYPS ELFTP ECKFK ESVFE NYYVI YSSML YRQQE SGRAW FLGLN KEGQA MKGNR VKKTK PAAHF LPKPL EVAMY REPSL HDVGE TVPKP GVTPS KSTSA SAIMN GGKPV NKSKT with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 21 ng/ml. |
| Alternate Names | FHF4; FHF-4; SCA27; Fibroblast growth factor 14; FGF-14; Fibroblast growth factor homologous factor 4 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF14 |
|---|---|
| Gene Full Name | fibroblast growth factor 14 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A mutation in this gene is associated with autosomal dominant cerebral ataxia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Probably involved in nervous system development and function. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
