ARG70122
Human FGF16 recombinant protein (Active) (His-tagged, C-ter)
Human FGF16 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF16 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF16 |
| Species | Human |
| A.A. Sequence | MAEVG GVFAS LDWDL HGFSS SLGNV PLADS PGFLN ERLGQ IEGKL QRGSP TDFAH LKGIL RRRQL YCRTG FHLEI FPNGT VHGTR HDHSR FGILE FISLA VGLIS IRGVD SGLYL GMNER GELYG SKKLT RECVF REQFE ENWYN TYAST LYKHS DSERQ YYVAL NKDGS PREGY RTKRH QKFTH FLPRP V DPS KLPSM SR DL FHYR with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 31 ng/ml. The specific activity of recombinant human FGF-16 is > 3 x 10^4 IU/mg. |
| Alternate Names | FGF-16; Fibroblast growth factor 16; MF4 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF16 |
|---|---|
| Gene Full Name | fibroblast growth factor 16 |
| Background | This gene encodes a member of a family of proteins that are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene is expressed in cardiac cells and is required for proper heart development. Mutation in this gene was also observed in individuals with metacarpal 4-5 fusion. [provided by RefSeq, Mar 2014] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
