ARG70124
Human FGF18 recombinant protein (Active) (His-tagged, C-ter)
Human FGF18 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF18 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF18 |
| Species | Human |
| A.A. Sequence | MAEEN VDFRI HVENQ TRARD DVSRK QLRLY QLYSR TSGKH IQVLG RRISA RGEDG DKYAQ LLVET DTFGS QVRIK GKETE FYLCM NRKGK LVGKP DGTSK ECVFI EKVLE NNYTA LMSAK YSGWY VGFTK KGRPR KGPKT RENQQ DVHFM KRYPK GQPEL QKPFK YTTVT KRSR with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 1.3-2.0 ng/ml. The specific activity of recombinant human FGF-18 is > 5 x 10^5 IU/mg. |
| Alternate Names | ZFGF5; Fibroblast growth factor 18; zFGF5; FGF-18 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF18 |
|---|---|
| Gene Full Name | fibroblast growth factor 18 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
