ARG70125
Human FGF19 recombinant protein (Active) (His-tagged, C-ter)
Human FGF19 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF19 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF19 |
| Species | Human |
| A.A. Sequence | MRPLA FSDAG PHVHY GWGDP IRLRH LYTSG PHGLS SCFLR IRADG VVDCA RGQSA HSLLE IKAVA LRTVA IKGVH SVRYL CMGAD GKMQG LLQYS EEDCA FEEEI RPDGY NVYRS EKHRL PVSLS SAKQR QLYKN RGFLP LSHFL PMLPM VPEEP EDLRG HLESD MFSSP LETDS MDPFG LVTGL EAVRS PSFEK with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 51 ng/ml. |
| Alternate Names | Fibroblast growth factor 19; FGF-19 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF19 |
|---|---|
| Gene Full Name | fibroblast growth factor 19 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008] |
| Function | Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
