ARG70126
Human FGF20 recombinant protein (Active) (His-tagged, C-ter)
Human FGF20 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF20 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF20 |
| Species | Human |
| A.A. Sequence | MPLAE VGGFL GGLEG LGQQV GSHFL LPPAG ERPPL LGERR SAAER SARGG PGAAQ LAHLH GILRR RQLYC RTGFH LQILP DGSVQ GTRQD HSLFG ILEFI SVAVG LVSIR GVDSG LYLGM NDKGE LYGSE KLTSE CIFRE QFEEN WYNTY SSNIY KHGDT GRRYF VALNK DGTPR DGARS KRHQK FTHFL PRPVD PERVP ELYKD LLMYT with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 1.3-3.2 ng/ml. The specific activity of recombinant human FGF-20 is > 2 x 10^5 IU/mg. |
| Alternate Names | Fibroblast growth factor 20; FGF-20; RHDA2 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF20 |
|---|---|
| Gene Full Name | fibroblast growth factor 20 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor family. The fibroblast growth factors possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene product is a secreted neurotrophic factor but lacks a typical signal peptide. It is expressed in normal brain, particularly the cerebellum, and may regulate central nervous system development and function. Homodimerization of this protein was shown to regulate its receptor binding activity and concentration gradient in the extracellular matrix. Genetic variations of this gene have been associated with Parkinson disease susceptibility. [provided by RefSeq, Oct 2009] |
| Function | Neurotrophic factor that regulates central nervous development and function. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
