ARG80158

Human FGF21 ELISA Kit

Human FGF21 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80158-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80158-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80158-003 Standard diluent buffer 20 ml 4°C
ARG80158-004 Antibody conjugate concentrate 400 µl 4°C
ARG80158-005 Antibody diluent buffer 16 ml 4°C
ARG80158-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80158-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80158-008 20X Wash buffer 50 ml 4°C
ARG80158-009 TMB substrate 12ml 4°C (Protect from light)
ARG80158-010 STOP solution 12ml 4°C
ARG80158-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80158 Human FGF21 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF21 in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human FGF-19, FGF-23, FGF R1 (IIIb)/Fc Chimera, FGF R1 (IIIc)/Fc Chimera, FGF R2 (IIIb)/Fc Chimera, FGF R2 (IIIc)/Fc Chimera, Klotho;mouse FGF R2 (IIIb)/Fc Chimera, FGF R2 (IIIc)/Fc Chimera
Target Name FGF21
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 31.3 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names Fibroblast growth factor 21; FGF-21

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26291 Human FGF21

Swiss-port # Q9NSA1 Human Fibroblast growth factor 21

Gene Symbol FGF21
Gene Full Name fibroblast growth factor 21
Background FGF-21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes. Recombinant human FGF-21 is a 19.5 kDa protein containing 182 amino acid residues.
Highlight Related products:
FGF21 antibodies; FGF21 ELISA Kits; FGF21 recombinant proteins;
Related news:
A new role of fat – factory of circulating miRNAs
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Title Download Link
ARG80158 Human FGF21 ELISA Kit User's manual download icon Download