ARG70127
Human FGF21 recombinant protein (Active) (His-tagged, C-ter)
Human FGF21 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF21 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF21 |
| Species | Human |
| A.A. Sequence | MHPIP DSSPL LQFGG QVRQR YLYTD DAQQT EAHLE IREDG TVGGA ADQSP ESLLQ LKALK PGVIQ ILGVK TSRFL CQRPD GALYG SLHFD PEACS FRELL LEDGY NVYQS EAHGL PLHLP GNKSP HRDPA PRGPA RFLPL PGLPP ALPEP PGILA PQPPD VGSSD PLSMV GPSQG RSPSY AS with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in BaF3 mouse pro-B cells transfected with human FGFRIIIc. The ED50 for this effect is < 0.4 μg/ml. |
| Alternate Names | Fibroblast growth factor 21; FGF-21 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF21 |
|---|---|
| Gene Full Name | fibroblast growth factor 21 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008] |
| Function | Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
