ARG70128
Human FGF22 recombinant protein (Active) (His-SUMO tagged, N-ter)
Human FGF22 recombinant protein (Active) (His-SUMO tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged and SUMO-tagged (N-ter) Active Human FGF22 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF22 |
| Species | Human |
| A.A. Sequence | TPSAS RGPRS YPHLE GDVRW RRLFS STHFF LRVDP GGRVQ GTRWR HGQDS ILEIR SVHVG VVVIK AVSSG FYVAM NRRGR LYGSR LYTVD CRFRE RIEEN GHNTY ASQRW RRRGQ PMFLA LDRRG GPRPG GRTRR YHLSA HFLPV LVS with polyhistidine tag and SUMO tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2 ng/ml. |
| Alternate Names | Fibroblast growth factor 22; FGF-22 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF22 |
|---|---|
| Gene Full Name | fibroblast growth factor 22 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | Plays a role in the fasting response, glucose homeostasis, lipolysis and lipogenesis. Can stimulate cell proliferation (in vitro). May be involved in hair development. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
