ARG70110
Human FGF3 recombinant protein (Active) (His-tagged, C-ter)
Human FGF3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF3 |
| Species | Human |
| A.A. Sequence | MDAGG RGGVY EHLGG APRRR KLYCA TKYHL QLHPS GRVNG SLENS AYSIL EITAV EVGIV AIRGL FSGRY LAMNK RGRLY ASEHY SAECE FVERI HELGY NTYAS RLYRT VSSTP GARRQ PSAER LWYVS VNGKG RPRRG FKTRR TQKSS LFLPR VLDHR DHEMV RQLQS GLPRP PGKGV QPRRR R with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 78 ng/ml. |
| Alternate Names | FGF-3; Fibroblast growth factor 3; Heparin-binding growth factor 3; Proto-oncogene Int-2; INT2; HBGF-3 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF3 |
|---|---|
| Gene Full Name | fibroblast growth factor 3 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal ear development. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
