ARG70112
Human FGF5 recombinant protein (Active) (His-tagged, C-ter)
Human FGF5 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF5 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF5 |
| Species | Human |
| A.A. Sequence | MAWAH GEKRL APKGQ PGPAA TDRNP IGSSS RQSSS SAMSS SSASS SPAAS LGSQG SGLEQ SSFQW SPSGR RTGSL YCRVG IGFHL QIYPD GKVNG SHEAN MLSVL EIFAV SQGIV GIRGV FSNKF LAMSK KGKLH ASAKF TDDCK FRERF QENSY NTYAS AIHRT EKTGR EWYVA LNKRG KAKRG CSPRV KPQHI STHFL PRFKQ SEQPE LSFTV TVPEK KNPPS PIKSK IPLS APRKN T NSV KYRLK FR FG with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.7 ng/ml. The specific activity of recombinant human FGF-5 is > 1.4 x 10^6 IU/mg. |
| Alternate Names | FGF-5; TCMGLY; Fibroblast growth factor 5; Heparin-binding growth factor 5; Smag-82; HBGF-5 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF5 |
|---|---|
| Gene Full Name | fibroblast growth factor 5 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified as an oncogene, which confers transforming potential when transfected into mammalian cells. Targeted disruption of the homolog of this gene in mouse resulted in the phenotype of abnormally long hair, which suggested a function as an inhibitor of hair elongation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
