ARG70113
Human FGF6 recombinant protein (Active) (His-tagged, C-ter)
Human FGF6 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF6 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF6 |
| Species | Human |
| A.A. Sequence | MGTRA NNTLL DSRGW GTLLS RSRAG LAGEI AGVNW ESGYL VGIKR QRRLY CNVGI GFHLQ VLPDG RISGT HEENP YSLLE ISTVE RGVVS LFGVR SALFV AMNSK GRLYA TPSFQ EECKF RETLL PNNYN AYESD LYQGT YIALS KYGRV KRGSK VSPIM TVTHF LPRI with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.1 ng/ml. The specific activity of recombinant human FGF-6 is > 1 x 10^7 IU/mg |
| Alternate Names | HST-2; HSTF-2; FGF-6; HST2; Heparin secretory-transforming protein 2; Fibroblast growth factor 6; Heparin-binding growth factor 6; HBGF-6 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF6 |
|---|---|
| Gene Full Name | fibroblast growth factor 6 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration. [UniProt] |
| Cellular Localization | Secreted, extracellular space. [UniProt] |
