ARG70115
Human FGF9 recombinant protein (Active) (His-tagged, C-ter)
Human FGF9 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF9 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF9 |
| Species | Human |
| A.A. Sequence | MPLGE VGNYF GVQDA VPFGN VPVLP VDSPV LLSDH LGQSE AGGLP RGPAV TDLDH LKGIL RRRQL YCRTG FHLEI FPNGT IQGTR KDHSR FGILE FISIA VGLVS IRGVD SGLYL GMNEK GELYG SEKLT QECVF REQFE ENWYN TYSSN LYKHV DTGRR YYVAL NKDGT PREGT RTKRH QKFTH FLPRP VDPDK VPELY K DIL SQS with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2 ng/ml. |
| Alternate Names | Fibroblast growth factor 9; Glia-activating factor; FGF-9; HBFG-9; HBGF-9; GAF; Heparin-binding growth factor 9; SYNS3 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF9 |
|---|---|
| Gene Full Name | fibroblast growth factor 9 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step. N-glycosylated. [UniProt] |
