ARG70104
Human FLT3 Ligand recombinant protein (Active) (His-tagged, C-ter)
Human FLT3 Ligand recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FLT3 Ligand recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FLT3 Ligand |
| Species | Human |
| A.A. Sequence | MTQDC SFQHS PISSD FAVKI RELSD YLLQD YPVTV ASNLQ DEELC GGLWR LVLAQ RWMER LKTVA GSKMQ GLLER VNTEI HFVTK CAFQP PPSCL RFVQT NISRL LQETS EQLVA LKPWI TRQNF SRCLE LQCQP DSSTL PPPWS PRPLE ATAPT A with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is < 0.8 ng/ml. The specific activity of recombinant human Flt-3 Ligand is > 1.5 x 10^6 IU/mg. |
| Alternate Names | FLT3L; SL cytokine; Fms-related tyrosine kinase 3 ligand; Flt3L; Flt3 ligand; FL |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FLT3LG |
|---|---|
| Gene Full Name | fms-related tyrosine kinase 3 ligand |
| Background | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011] |
| Function | Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
