ARG82722

Human FUT8 ELISA Kit

Human FUT8 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82722-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82722-002 Standard 2 X 10 ng/vial 4°C
ARG82722-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82722-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82722-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82722-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82722-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82722-008 25X Wash buffer 20 ml 4°C
ARG82722-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82722-010 STOP solution 10 ml (Ready to use) 4°C
ARG82722-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82722 Human FUT8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FUT8 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name FUT8
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 6.9%
Alternate Names Alpha-(1,6)-fucosyltransferase; Alpha1-6FucT; EC 2.4.1.68; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2530 Human FUT8

Swiss-port # Q9BYC5 Human Alpha-(1,6)-fucosyltransferase

Gene Symbol FUT8
Gene Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Background This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]
Function Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. [UniProt]
Cellular Localization Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt]
Highlight Related products:

New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Tyrosine phosphorylated by PKDCC/VLK. [UniProt]
Title Download Link
ARG82722 Human FUT8 ELISA kit User manual download icon Download