ARG81519

Human Factor D ELISA Kit

Human Factor D ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81519-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81519-002 Standard 2 X 20 ng/vial 4°C
ARG81519-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81519-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81519-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81519-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81519-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81519-008 25X Wash buffer 20 ml 4°C
ARG81519-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81519-010 STOP solution 10 ml (Ready to use) 4°C
ARG81519-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81519 Human Factor D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor D in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Factor D
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 6.1%
Alternate Names C3 convertase activator; PFD; Properdin factor D; DF; Complement factor D; ADIPSIN; EC 3.4.21.46; ADN; Adipsin

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1675 Human CFD

Swiss-port # P00746 Human Complement factor D

Gene Symbol CFD
Gene Full Name complement factor D (adipsin)
Background The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]
Function Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. [UniProt]
Highlight Related products:
Factor D antibodies; Factor D ELISA Kits; Factor D Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81519 Human Factor D ELISA Kit User's manual download icon Download