ARG81109

Human Factor IX (total) ELISA Kit

Human Factor IX (total) ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit

Overview

Product Description ARG81109 Human Factor IX (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of  Human Factor IX (total) in plasma.
Tested Reactivity Hu
Tested Application ELISA
Target Name Factor IX
Conjugation HRP
Conjugation Note TMB substrate is used for color development at 450 nm.
Sensitivity 0.012 ng/ml
Sample Type Plasma
Standard Range 0.1 - 100 ng/ml
Alternate Names Coagulation factor IX; HEMB; FIX; PTC; Plasma thromboplastin component; F9 p22; THPH8; EC 3.4.21.22; P19; Christmas factor

Properties

Form 96 well
Storage Instruction Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2158 Human F9

Swiss-port # P00740 Human Coagulation factor IX

Gene Symbol F9
Gene Full Name coagulation factor IX
Background This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]
Function Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. [UniProt]
Highlight Related products:
Factor IX antibodies; Factor IX ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Immune System kit
PTM Activated by factor XIa, which excises the activation peptide (PubMed:9169594, PubMed:1730085). The propeptide can also be removed by snake venom protease (PubMed:20004170, PubMed:20080729).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro. Xylosylation at this site is minor.
Title Download Link
ARG81109 Human Factor IX (total) ELISA Kit User's manual download icon Download