ARG81113
Human Factor XII (total) ELISA Kit
Human Factor XII (total) ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Immune System kit
Overview
| Product Description | ARG81113 Human Factor XII (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor XII (total) in plasma. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Factor XII |
| Conjugation | HRP |
| Conjugation Note | TMB substrate is used for color development at 450 nm. |
| Sensitivity | 0.137 ng/ml |
| Sample Type | Plasma |
| Standard Range | 0.5 - 100 ng/ml |
| Alternate Names | Hageman factor; Coagulation factor XII; HAF; EC 3.4.21.38; HAEX; Beta-factor XIIa part 2; HAE3 |
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F12 |
| Gene Full Name | coagulation factor XII (Hageman factor) |
| Background | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008] |
| Function | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. [UniProt] |
| Highlight | Related products: Factor XII antibodies; Factor XII ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit |
| PTM | Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. |
| Title | Download Link |
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| ARG81113 Human Factor XII (total) ELISA Kit |
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