ARG81134

Human Factor XIII ELISA Kit

Human Factor XIII ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81134-001 Antibody-coated Microtiter Plate 12 x 8 strips 4°C
ARG81134-002 20X wash buffer concentrate 30ml X 2 bottles 4°C
ARG81134-003 Plate sealer 3 pieces 4°C
ARG81134-004 Biotinylated Human Factor XIII antibody (50X) 1 vial (140 μl) 4°C
ARG81134-005 Human FXIII Standard 1 vial (160ng, lyophilized) 4°C
ARG81134-006 10X EIA Diluent 30 ml 4°C
ARG81134-007 100X Streptavidin-HRP 80 μl 4°C
ARG81134-008 TMB Substrate 8 ml 4°C, ready for use
ARG81134-009 STOP solution 12 ml 4°C, ready for use

Overview

Product Description

ARG81134 Human Factor XIII ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor XIII in serum, plasma, cell culture, urine, saliva, milk and CSF.

Tested Reactivity Hu
Tested Application ELISA
Target Name Factor XIII
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 2.1 ng/ml
Sample Type Serum, plasma, cell culture, urine, saliva, milk and CSF
Standard Range 2.5 - 160 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: 4.4%
Inter-Assay CV: 9.4%
Alternate Names Coagulation factor XIIIa; F13A; Protein-glutamine gamma-glutamyltransferase A chain; Coagulation factor XIII A chain; Transglutaminase A chain; EC 2.3.2.13

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2162 Human F13A1

Swiss-port # P00488 Human Coagulation factor XIII A chain

Gene Symbol F13A1
Gene Full Name coagulation factor XIII, A1 polypeptide
Background This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]
Function Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. [UniProt]
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PTM The activation peptide is released by thrombin.
Title Download Link
ARG81134 Human Factor XIII ELISA Kit User's manual download icon Download