ARG81500
Human Fibroblast activation protein ELISA Kit
Human Fibroblast activation protein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81500-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81500-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81500-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81500-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81500-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81500-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81500-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81500-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81500-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81500-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81500-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81500 Human Fibroblast activation protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Fibroblast activation protein in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Fibroblast activation protein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| Alternate Names | Fibroblast activation protein alpha; SIMP; Seprase; Integral membrane serine protease; Surface-expressed protease; EC 3.4.21.26; Dipeptidyl peptidase FAP; 170 kDa melanoma membrane-bound gelatinase; EC 3.4.14.5; APCE; Serine integral membrane protease; DPPIV; EC 3.4.21.-; FAPalpha; Gelatine degradation protease FAP; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; FAPA |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FAP |
| Gene Full Name | fibroblast activation protein, alpha |
| Background | The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
| Function | Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner. [UniProt] |
| Highlight | Related products: Fibroblast activation protein antibodies; Fibroblast activation protein ELISA Kits; Fibroblast activation protein Duos / Panels; Related news: New antibody panels for Myofibroblasts and CAFs New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. The N-terminus may be blocked. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81500 Human Fibroblast activation protein ELISA Kit User's manual |
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