ARG81409
Human Flt3 Ligand ELISA Kit
Human Flt3 Ligand ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81409-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81409-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81409-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81409-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81409-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81409-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81409-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81409-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81409-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81409-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81409-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81409 Human Flt3 Ligand ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Flt3 Ligand in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FLT3 Ligand |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.1% Inter-Assay CV: 8.4% |
| Alternate Names | FLT3L; SL cytokine; Fms-related tyrosine kinase 3 ligand; Flt3L; Flt3 ligand; FL |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49771 Human Fms-related tyrosine kinase 3 ligand |
|---|---|
| Gene Symbol | FLT3LG |
| Gene Full Name | fms-related tyrosine kinase 3 ligand |
| Background | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011] |
| Function | Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [UniProt] |
| Highlight | Related products: FLT3 Ligand antibodies; FLT3 Ligand ELISA Kits; FLT3 Ligand recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81409 Human Flt3 Ligand ELISA Kit User's manual |
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