ARG70289

Human G-CSF Receptor recombinant protein (Active) (ECD) (His-tagged, C-ter)

Human G-CSF Receptor recombinant protein (Active) (ECD) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human G-CSF Receptor recombinant protein (ECD).
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name G-CSF Receptor (ECD)
Species Human
A.A. Sequence Glu25 - Pro621 of Human G-CSF Receptor (NP_000751.1) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured by its ability to inhibit the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this ettect is typically 31.5-126 ng/ml.
Alternate Names G-CSF receptor; CD114; CD antigen CD114; G-CSF-R; GCSFR; Granulocyte colony-stimulating factor receptor

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1441 Human CSF3R

Swiss-port # Q99062 Human Granulocyte colony-stimulating factor receptor

Gene Symbol CSF3R
Gene Full Name colony stimulating factor 3 receptor (granulocyte)
Background The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]
Function Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. [UniProt]
Cellular Localization Isoform 2: Secreted. Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 92 kDa
PTM N-glycosylated. [UniProt]