ARG70098
Human GDF7 recombinant protein (Active) (His-tagged, C-ter)
Human GDF7 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human GDF7 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GDF7 |
| Species | Human |
| A.A. Sequence | MTALA GTRTA QGSGG GAGRG HGRRG RSRCS RKPLH VDFKE LGWDD WIIAP LDYEA YHCEG LCDFP LRSHL EPTNH AIIQT LLNSM APDAA PASCC VPARL SPISI LYIDA ANNVV YKQYE DMVVE ACGCR with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 112 ng/ml. |
| Alternate Names | Growth/differentiation factor 7; BMP12; GDF-7 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GDF7 |
|---|---|
| Gene Full Name | growth differentiation factor 7 |
| Background | This gene encodes a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord. [provided by RefSeq, Jul 2008] |
| Function | May play an active role in the motor area of the primate neocortex. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
