ARG70143
Human GDNF recombinant protein (Active) (His-tagged, C-ter)
Human GDNF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human GDNF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GDNF |
| Species | Human |
| A.A. Sequence | MSPDK QMAVL PRRER NRQAA AANPE NSRGK GRRGQ RGKNR GCVLT AIHLN VTDLG LGYET KEELI FRYCS GSCDA AETTY DKILK NLSRN RRLVS DKVGQ ACCRP IAFDD DLSFL DDNLV YHILR KHSAK RCGCI with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is < 10 ng/ml. |
| Alternate Names | ATF; Astrocyte-derived trophic factor; HSCR3; hGDNF; Glial cell line-derived neurotrophic factor; HFB1-GDNF; ATF1; ATF2 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GDNF |
|---|---|
| Gene Full Name | glial cell derived neurotrophic factor |
| Background | This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010] |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
