ARG82853
Human GSTA1 ELISA Kit
Human GSTA1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82853-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82853-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82853-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82853-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82853-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82853-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82853-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82853-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82853-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82853-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82853-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82853 Human GSTA1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GSTA1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | GSTA1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.8% |
| Alternate Names | EC 2.5.1.18; Glutathione S-transferase A1; GSTA1-1; GST-epsilon; GST class-alpha member 1; GTH1; GST2; GST HA subunit 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GSTA1 |
| Gene Full Name | glutathione S-transferase alpha 1 |
| Background | This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Function | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82853 Human GSTA1 ELISA Kit User manual |
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