ARG82609
Human Galactosidase alpha ELISA Kit
Human Galactosidase alpha ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82609 Human Galactosidase alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Galactosidase alpha in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human Galactosidase alpha. No significant cross-reactivity or interference was observed in the following samples: Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. |
| Target Name | Galactosidase alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 250 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 500 - 32000 pg/ml |
| Sample Volume | 20 µl |
| Precision | Intra-Assay CV: 6.9% Inter-Assay CV: 5.8% |
| Alternate Names | Alpha-galactosidase A; Melibiase; Alpha-D-galactosidase A; Alpha-D-galactoside galactohydrolase; EC 3.2.1.22; Agalsidase; GALA |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GLA |
| Gene Full Name | galactosidase, alpha |
| Background | This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: Galactosidase alpha antibodies; Galactosidase alpha ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG82609 Human Galactosidase alpha ELISA Kit User manual |
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