ARG70146
Human Galectin 1 recombinant protein (Active) (His-tagged, N-ter)
Human Galectin 1 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human Galectin 1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Galectin 1 |
| Species | Human |
| A.A. Sequence | ACGLV ASNLN LKPGE CLRVR GEVAP DAKSF VLNLG KDSNN LCLHF NPRFN AHGDA NTIVC NSKDG GAWGT EQREA VFPFQ PGSVA EVCIT FDQAN LTVKL PDGYE FKFPN RLNLE AINYM AADGD FKIKC VAFD with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is < 2 μg/ml. |
| Alternate Names | HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LGALS1 |
|---|---|
| Gene Full Name | lectin, galactoside-binding, soluble, 1 |
| Background | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008] |
| Function | May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
