ARG70148
Human Galectin 4 recombinant protein (Active) (His-tagged, N-ter)
Human Galectin 4 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human Galectin 4 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Galectin 4 |
| Species | Human |
| A.A. Sequence | AYVPA PGYQP TYNPT LPYYQ PIPGG LNVGM SVYIQ GVASE HMKRF FVNFV VGQDP GSDVA FHFNP RFDGW DKVVF NTLQG GKWGS EERKR SMPFK KGAAF ELVFI VLAEH YKVVV NGNPF YEYGH RLPLQ MVTHL QVDGD LQLQS INFIG GQPLR PQGPP MMPPY PGPGH CHQQL NSLPT MEGPP TFNPP VPYFG RLQGG LTARR TIIIK GYVPP TGKSF AINFK VGSSG DIALH INPRM GNGTV VRNSL LNGSW GSEEK KITHN PFGPG QFFDL SIRCG LDRFK VYANG QHLFD FAHRL SAFQR VDTLE IQGDV TLSYV QI with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is < 8 μg/ml. |
| Alternate Names | GAL4; L36LBP; L-36 lactose-binding protein; Gal-4; Galectin-4; Lactose-binding lectin 4; Antigen NY-CO-27 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LGALS4 |
|---|---|
| Gene Full Name | lectin, galactoside-binding, soluble, 4 |
| Background | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008] |
| Function | Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. [UniProt] |
