ARG70149
Human Galectin 7 recombinant protein (Active) (His-tagged, N-ter)
Human Galectin 7 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human Galectin 7 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Galectin 7 |
| Species | Human |
| A.A. Sequence | SNVPH KSSLP EGIRP GTVLR IRGLV PPNAS RFHVN LLCGE EQGSD AALHF NPRLD TSEVV FNSKE QGSWG REERG PGVPF QRGQP FEVLI IASDD GFKAV VGDAQ YHHFR HRLPL ARVRL VEVGG DVQLD SVRIF with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is < 2 μg/ml. |
| Alternate Names | GAL7; Gal-7; HKL-14; LGALS7A; Galectin-7; p53-induced gene 1 protein; PI7 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LGALS7 |
|---|---|
| Gene Full Name | lectin, galactoside-binding, soluble, 7 |
| Background | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008] |
| Function | Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Secreted. Note=May be secreted by a non-classical secretory pathway. [UniProt] |
