ARG82773
Human Ghrelin (total) ELISA Kit
Human Ghrelin (total) ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82773 Human Ghrelin (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Ghrelin (total) in serum and plasma. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross Reactivity (peptide): Human Ghrelin: 100% Rat Ghrelin: 100% Human Ghrelin 1-27 (O-n-Octanoyl-Ser3): 100% Human Ghrelin 1-27 (O-n-Decanoyl-Ser3): 100% Human Ghrelin (Des-Octanoyl-Ser3): 100% Rat Ghrelin (Des-Octanoyl-Ser3): 100% C-Terminal of Ghrelin: 100% Human Ghrelin (Gln28): 100% Monkey Ghrelin: 85% Pig Ghrelin, Human Secretin, Human VIP, Pig VIP, Rat VIP, Rat Prolactin-Releasing Peptide-31, Human Prolactin-Releasing Peptide-31, Rat Galanin, Human Galanin, Rat GH-RF, Human GH-RF, Human NPY, Human Orexin A, Bovine Orexin A, Mouse Orexin A, Rat Orexin A and Human Orexin B: 0% |
| Target Name | Ghrelin (total) |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.13 ng/ml |
| Detection Range | Linear range: 0.13 - 1.34 ng/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 0.01 - 100 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 15% |
| Alternate Names | Motilin-related peptide; Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; MTLRP; Ghrelin; Protein M46 |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GHRL |
| Gene Full Name | ghrelin/obestatin prepropeptide |
| Background | This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014] |
| Function | Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid (PubMed:10604470). Amidation of Leu-98 is essential for obestatin activity. [UniProt] |
| Title | Download Link |
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| ARG82773 Human Ghrelin (total) ELISA Kit User manual |
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