ARG82383

Human Glypican 5 ELISA Kit

Human Glypican 5 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82383-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82383-002 Standard 2 X 10 ng/vial 4°C
ARG82383-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82383-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82383-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82383-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82383-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82383-008 25X Wash buffer 20 ml 4°C
ARG82383-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82383-010 STOP solution 10 ml (Ready to use) 4°C
ARG82383-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82383 Human Glypican 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Glypican 5 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Glypican 5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%
Inter-Assay CV: 7.0%
Alternate Names Glypican-5

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2262 Human GPC5

Swiss-port # P78333 Human Glypican-5

Gene Symbol GPC5
Gene Full Name glypican 5
Background Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]
Function Cell surface proteoglycan that bears heparan sulfate. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted glypican-5: Secreted, extracellular space. [UniProt]
Highlight Related products:
Glypican antibodies; Glypican ELISA Kits;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG82383 Human Glypican 5 ELISA Kit User manual download icon Download