ARG81454
Human HB EGF ELISA Kit
Human HB EGF ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81454-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81454-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81454-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81454-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81454-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81454-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81454-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81454-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81454-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81454-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81454-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81454 Human HB EGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HB EGF in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HB EGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 5.7% |
| Alternate Names | Diphtheria toxin receptor; HBEGF; HB-EGF; HEGFL; Proheparin-binding EGF-like growth factor; DTSF; DT-R; DTS; DTR |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99075 Human Proheparin-binding EGF-like growth factor |
|---|---|
| Gene Symbol | HBEGF |
| Gene Full Name | heparin-binding EGF-like growth factor |
| Function | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. [UniProt] |
| Highlight | Related products: Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active. O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81454 Human HB EGF ELISA Kit User's manual |
 Download
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