ARG81356

Human HDGF ELISA Kit

Human HDGF ELISA Kit for ELISA and Human

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Overview

Product Description ARG81356 Human HDGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HDGF in serum, plasma and cell culture supernatant.
Tested Reactivity Hu
Tested Application ELISA
Target Name HDGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity ~ 30 pg/ml
Sample Type Serum, plasma and cell culture supernatant.
Standard Range 78 - 5000 pg/ml
Sample Volume 50 µl
Alternate Names HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3068 Human HDGF

Swiss-port # P51858 Human Hepatoma-derived growth factor

Gene Symbol HDGF
Gene Full Name hepatoma-derived growth factor
Background HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
Function HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618).

Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719).

Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt]
Highlight Related products:
HDGF antibodies; HDGF ELISA Kits;
Related news:
The most sensitive human HDGF ELISA kit
The role of HDGF in tumor angiogenesis
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. [UniProt]

Specific References

HDGF enhances VEGF-dependent angiogenesis and FGF-2 is a VEGF-independent angiogenic factor in non-small cell lung cancer.

ELISA / Human / Cell culture supernatant

Eguchi Ryoji et al.
Oncol Rep.,  (2020)

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HDGF supports anti-apoptosis and pro-fibrosis in pancreatic stellate cells of pancreatic cancer.

ELISA / Human

Chen Yi-Ting et al.
Preprint.,  (2018)

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Title Download Link
ARG81356 Human HDGF ELISA Kit User's manual download icon Download