ARG81734

Human HER2 / ErbB2 ELISA Kit

Human HER2 / ErbB2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81734-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81734-002 Standard 2 X 10 ng/vial 4°C
ARG81734-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81734-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81734-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81734-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81734-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81734-008 25X Wash buffer 20 ml 4°C
ARG81734-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81734-010 STOP solution 10 ml (Ready to use) 4°C
ARG81734-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81734 Human HER2 / ErbB2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HER2 / ErbB2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ERBB2 / HER2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.3 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.1%; Inter-Assay CV: 8.0%
Alternate Names p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2064 Human ERBB2

Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2

Gene Symbol ERBB2
Gene Full Name erb-b2 receptor tyrosine kinase 2
Background This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. [UniProt]
Highlight Related products:
ERBB2 antibodies; ERBB2 ELISA Kits; ERBB2 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). [UniProt]
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