ARG81739

Human HGFA Inhibitor 2 ELISA Kit

Human HGFA Inhibitor 2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81739-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81739-002 Standard 2 X 10 ng/vial 4°C
ARG81739-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81739-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81739-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81739-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81739-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81739-008 25X Wash buffer 20 ml 4°C
ARG81739-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81739-010 STOP solution 10 ml (Ready to use) 4°C
ARG81739-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81739 Human HGFA Inhibitor 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HGFA Inhibitor 2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name HGFA Inhibitor 2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%; Inter-Assay CV: 6.1%
Alternate Names HAI2; Kunitz-type protease inhibitor 2; PB; Placental bikunin; HAI-2; Hepatocyte growth factor activator inhibitor type 2; Kop; DIAR3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10653 Human SPINT2

Swiss-port # O43291 Human Kunitz-type protease inhibitor 2

Gene Symbol SPINT2
Gene Full Name serine peptidase inhibitor, Kunitz type, 2
Background This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Function Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. [UniProt]
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Title Download Link
ARG81739 Human HGFA Inhibitor 2 ELISA Kit User's manual download icon Download